Starting Monday, motorists who drive or stand in the bus lane along 125th Street in Manhattan during designated hours will be issued $115 fines.
The Department of Transportation has been handing out warnings for more than 60 days.
But beginning next week, the law will be strictly enforced with the help of cameras.
The corridors are used by the M60 Select Service Bus, which carries riders from LaGuardia Airport to Morningside Heights.
Transportation officials say 125th Street is one of upper Manhattan’s busiest commercial corridors, with over 30,000 weekday bus riders.