Preliminary tests show the 16-month-old Queens boy who died after being brought to a hospital with a fever earlier this week did not have the H1N1 virus.

The health department says it will now send tissue samples from the autopsy to the Centers for Disease Control and Prevention for further testing.

Results are expected later this week.

The boy, named Jonathan Zamora, was admitted to Elmhurst Hospital Monday night with a high fever and died within an hour.

"He had a fever in the morning...Through the day the baby was eating and at night just before 9:30, [his mother] brought him in," said Elmhurst Hospital spokesman Dario Centorcelli. "She checked on the child and he was unresponsive."

"He was fine. He was running. He was happy. Everything," said Zefarino Zamora, Jonathan's father, through a translator.

Zamora's three-year-old sister and one-year-old cousin were treated for flu-like symptoms and released, according to hospital sources.

Meanwhile, city officials on Tuesday confirmed three more city schools will shut their doors in response to a high number of flu-related absences. They include P.S. 130 in Chinatown, P.S. 35 in Jamaica and Merrick Academy Charter School in Queens Village.

Also on Tuesday, two Bronx schools -- South Bronx Charter School in Mott Haven and Horace Mann School in Riverdale -- and two Queens schools -- San Demetrios School in Astoria and Holy Family School in Flushing, announced plans to close.

All four schools are scheduled to reopen on Tuesday, May 26.

Officials at South Bronx Charter, which is made up of three campuses, have reported that many students have flu-like symptoms. Over 50 percent is the school's second grade is out sick and one student was hospitalized, according to the school's management.

Still, only one of the 23 closed city schools -- I.S. 238 in Hollis, Queens -- has confirmed cases of H1N1 virus.

Mayor Michael Bloomberg is continuing to stress the importance of hand washing and other precautions to limit the spread of the disease, but says anyone experiencing flu-like symptoms should seek medical attention.

"Let me stress just once again, whether you have health insurance coverage or your immigration status is in question, it doesn't matter, we will not ask about that," said the mayor. "I don't know and don't care what the immigration status of the child in question or his parents is, the only question is, 'Are you severely ill?'"

The Department of Health says there are now more than 190 confirmed cases of H1N1 citywide, but most cases have been no more severe than regular seasonal flu.

St. Joseph's School in Astoria, Queens decided to close Tuesday after 150 students called out sick, according to the Brooklyn Diocese. The school has not reported any cases of students with flu-like symptoms.

Department of Education officials are trying to determine whether the high number of school absences are due to genuine sickness or to parents keeping their children home from school.

Six more schools - four in Queens and two in Manhattan - decided to close Monday over concerns of spreading flu virus.

During a regularly scheduled meeting Tuesday between the Department of Education and the Public Education Panel, officials focused much of their discussions on the flu outbreak.

They say they are seeking a special waiver from the state so these schools don't have to make up missed days.

Officials also explained the many factors leading to the decision to close a school.

"It depends on the size of the school and the severity of the flu. It's not a numeric thing, it's a subjective thing where professionals make recommendations based on their judgement at the time," said Bloomberg.

"The problem that we're having now is that we have many schools with very nigh reports of illness but very little documentation in the medical room of children with what we call with influenza-like illness," said Dr. Roger Platt of the city Office of School Health.

Queens ER's See Steady Flow Of Patients

In Queens, hospitals are reporting a surge in emergency room visits from people complaining of flu-like symptoms.

The wait to see a doctor at several hospitals in the borough has been backed up for hours.

Queens Hospital Center in Jamaica was so overwhelmed with ER visitors Tuesday that workers set up a tent in the parking lot to handle patients.

The hospital has also been handing out surgical masks to people concerned about contracting the flu.

H1N1 Cases In Rikers Island Increase

On Tuesday, Mayor Bloomberg said there were four confirmed and four probable cases of H1N1 flu at Rikers Island.

The mayor said the jail cannot be evacuated, but steps are being taken to try and prevent the illness from spreading.

He also says every inmate who headed to court Tuesday was checked and that none have shown any flu symptoms.

President Norman Seabrook of the Correction Officers Benevolent Association, however, accused city officials today of not doing enough to prevent his members and the inmates from being infected.

"The inmates that are at Rikers Island do not have primary physicians that they go see every year," said Seabrook. "The inmates at Rikers Island do not go get a haircut or shave in the morning or wash the way they are supposed to. We are in an environment where this thing is going take legs and is going to take off."

Seabrook said his union plans to file a lawsuit against the city. He wants to force the Department of Corrections to take more measures to stop the virus from spreading through city jails.

On Saturday, Department of Health officials confirmed the first case at the jail, an inmate at the Ann M. Kross Center.

Over the weekend, visitors were not allowed to enter the affected quarters and officers were encouraged to wear masks, and the affected center was sanitized.

Queens School Remembers First H1N1 Fatality

On Monday, hundreds honored the life of a popular Queens assistant principal who was the first in the city to die of complications from the H1N1 virus.

A candlelight vigil was held outside I.S. 238 in Hollis to remember Mitchell Wiener, who died Sunday.

Wiener's wife of 28 years, Bonnie, who is a teacher at the school, said she's making peace with her loss and will be back in the classroom soon.

"It's been a nightmare that I thought I was going to be able to wake up from, but unfortunately, it's going to be my reality to be without him," said Bonnie Wiener. "[The vigil] does help, it does help because I know I'll have a life to come back to when I walk in from work when I finish my morning period, I'll come back here, I'll sit at his desk one more time, then go to my room and do my job."

Funeral services will be held Wednesday at 2 p.m. at Sinai Chapels at 162-05 Horace Harding Expressway in Flushing, Queens.

Four students in I.S. 238 also have confirmed cases of H1N1 flu.