About 40 accused members of the Bronx-based "Trinitarios" gang were indicted Wednesday.
They face charges ranging from murder and assault to racketeering and selling drugs.
27 alleged members are charged with nine separate murders in the Bronx and Yonkers.
Most of the victims were rival gang members, five of whom were teenagers at the time of their deaths.
"No one here wants to allow even one small step backward to the gang wars of the 1990s, and no one here wants our city to end up like some others that have recently seen startling spikes in violence because gangs have gone unchecked," said U.S. Attorney Preet Brahara.
Since 2009, 119 members of the Trinitarios gang have been accused of violent crimes and racketeering offenses.
Investigators say the gang was split into factions, including an all-female group known as the "Bad Barbies".