Investigators with the FBI and NYPD on Monday wrapped up their on-site search inside a SoHo building looking for clues in the disappearance of Etan Patz.
Officials say they did not find any human remains, but some items removed were sent to a Virginia crime lab for further testing.
Street and local businesses were reopened around the site.
Earlier Monday morning, two dumpsters outside 127B Prince Street holding excavated pieces of concrete and other items from the building's basement were picked up and hauled to Staten Island.
"I think it's important that every effort be made, and this is part of those efforts, even though it's a very old case. I think you have the expertise of two very good agencies - the FBI and NYPD - working on, making sure all efforts were made. And this was part of that effort," said Deputy Police Commissioner Paul Browne.
Sources tell NY1 another stain was found in the basement yesterday as agents searched for evidence in the case of the six-year-old who went missing in 1979.
Investigators on-site would not comment.
Etan's parents still live a half-block from the basement where investigators searched for evidence.
Residents who live in the neighborhood say the renewed search brings back memories of when the case first made headlines.
"Evil can happen anywhere it's just so sad the parents are like right down the block. You know to see it happen in your own neighborhood," said SoHo resident Linda Fanuele.
"We used to travel through the basements to get to one place to the other - just for fun - we used to play manhunt," said SoHo resident Benjamin Del Angel.
At the time Patz disappeared, the basement was being used by a handyman named Othniel Miller who was thought to be friendly with the boy.
His lawyer released a statement Monday that read, in part, "Mr. Miller and his family have been undeservedly dragged through the mud during this ordeal. The Miller family remains deeply saddened by what happened to young Etan Patz, and by the fact that he still has yet to be located. Mr. Miller, however, bears no responsibility for this tragedy."
No one has ever been prosecuted in the case. However, in a civil suit, convicted child molester Jose Ramos was found to be responsible.